We narrowed to 2,685 results for: T2A

1. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ-dSpRY-Act3.0

   Plasmid

   #158416

   Purpose

   CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LVDP 2P.2

   Plasmid

   #231904

   Purpose

   For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
   

   Use

   Lentiviral

   Mutation

   R203M in N protein

   Promoter

   CMV, EF-1a

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB9v1

   Plasmid

   #210028

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGLTR-X-GFP

   Plasmid

   #58245

   Purpose

   GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Inserts

   attR1-Cm(R)-CcdB-attR2
   TetR-NLS-T2A-GFP
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   GFP, NLS, T2A, and TetR

   Expression

   Mammalian

   Promoter

   SFFV and no

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ-dpcoCas9-Act3.0

   Plasmid

   #158408

   Purpose

   CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB9v2

   Plasmid

   #210029

   Purpose

   Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LVDP 2P.2.EGFP

    Plasmid

    #231905

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    

    Use

    Lentiviral

    Mutation

    R203M in N protein

    Promoter

    CMV, EF-1a

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. STEME-4

    Plasmid

    #138140

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
    

    Expression

    Plant

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. STEME-3

    Plasmid

    #138139

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
    

    Expression

    Plant

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCol.2lox4F2A targeting construct

    Plasmid

    #25795

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Nat Methods. 2010 Jan . 7(1):56-9.

    Insert

    loxP-Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc-loxP
    

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEntry Tshld-GFP

    Plasmid

    #53211

    Purpose

    pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

    Depositor

    Matthew Pratt

    Article

    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

    Insert

    Tshld-GFP
    

    Tags

    EGFP, Flag, and HA

    Promoter

    none

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-CMV5-N-cG-P

    Plasmid

    #192883

    Purpose

    coexpression of Nanoluc, copGFP and puromycin resistant marker

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-TO-hNIL BSD-mApple

    Plasmid

    #182313

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaRSC

    Plasmid

    #89189

    Purpose

    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ10704

    Plasmid

    #183957

    Purpose

    U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    SKSKSO array
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-TagBFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge