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pLenti Lifeact-iRFP670 BlastR

Plasmid

#84385

Purpose

Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Publication

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

iRFP670

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGAMA-YAP

Plasmid

#74942

Purpose

Lentiviral over-expression vector for YAP

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

T2A-mCherry

Promoter

EF1α

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDONR223-AURKB

Plasmid

#23682

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAd nGFP

Plasmid

#19413

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

nuclear GFP

Use

Adenoviral and Gateway Cloning

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Publication

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Tags

8xHIS and BPNLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR207 SARS-CoV-2 NSP1

Plasmid

#141255

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Publication

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Publication

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST 3XFlag-SRSF3

Plasmid

#46736

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

SRSF3 (SRSF3 Human)

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Publication

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

Plasmid

#104567

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain

Use

CRISPR

Tags

NLS-3xHA-VPR

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-PBRM1

Plasmid

#65387

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PBRM1 (PBRM1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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