We narrowed to 8,506 results for: sgrna

1. pU6-sp-sgRNA-mDNMT1_nick-lenti

   Plasmid

   #135959

   Purpose

   S. pyogenes nicking sgRNA for mouse DNMT1

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   mDNMT1_nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHKMC1: Empty sgRNA for Cloning

   Plasmid

   #67720

   Purpose

   Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

   Depositor

   Monica Colaiacovo

   Article

   Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   PU6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

   Plasmid

   #154197

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC2-A, sgRNA-BC2-C
   

   Use

   Lentiviral; Catch barcode activation

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and mU6

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

   Plasmid

   #154002

   Purpose

   Empty SgRNA expression system matching CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   SgRNA expression system
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, EF1a

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLb-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107682

   Purpose

   Expresses LbCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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