Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 6,271 results for: chia

Showing: 4401 - 4450 of 6271 results

  14. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid
    #238344
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96E; R103E; R105E
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238344-plasmid-map-sequence-id-472712

    Enlarge

  15. Ec FkP-dnaN(3A's)-pRSFDuet

    Plasmid
    #238343
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96A; R103A; R105A
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238343-plasmid-map-sequence-id-472707

    Enlarge

  16. Ec FkP-dnaN(3S's)-pRSFDuet

    Plasmid
    #238345
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96S; R103S; R105S
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238345-plasmid-map-sequence-id-472709

    Enlarge

  17. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Use
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

    Enlarge

  18. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

    Enlarge

  19. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

    Enlarge

  20. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid
    #83397
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83397-plasmid-map-sequence-id-175072

    Enlarge

  21. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

    Enlarge

  22. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

    Enlarge

  23. pSB3

    Plasmid
    #82027
    Purpose
    Hybrid McpC[1-350]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    82027-plasmid-map-sequence-id-175680

    Enlarge

  24. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

    Enlarge

  25. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

    Enlarge

  26. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

    Enlarge

  27. pSB2

    Plasmid
    #82026
    Purpose
    Hybrid McpC[1-286]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-286]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82026-plasmid-map-sequence-id-174389

    Enlarge

  28. pSB6

    Plasmid
    #82028
    Purpose
    Hybrid McpC[1-350]-YSYEH-Tar[272-553]
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-YSYEH-Tar[272-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82028-plasmid-map-sequence-id-174383

    Enlarge

  29. pSB10

    Plasmid
    #82039
    Purpose
    Hybrid McpS[1-295]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. putida chemoreceptor McpS: McpS[1-295]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82039-plasmid-map-sequence-id-174336

    Enlarge

  30. pSB8

    Plasmid
    #82037
    Purpose
    Hybrid CitA[1-193]-Tar[204-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82037-plasmid-map-sequence-id-174335

    Enlarge

  31. pSB11

    Plasmid
    #82040
    Purpose
    Hybrid NarQ[1-217]-Tar[257-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82040-plasmid-map-sequence-id-174334

    Enlarge

  32. pSB7

    Plasmid
    #82029
    Purpose
    Hybrid Tar[1-43]-CitA[55-173]-Tar[184-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82029-plasmid-map-sequence-id-174333

    Enlarge

  33. pSB9

    Plasmid
    #82038
    Purpose
    Hybrid ECA0434[1-385]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. atrosepticum chemoreceptor ECA0434: ECA0434[1-385]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82038-plasmid-map-sequence-id-174332

    Enlarge

  39. pHL 363

    Plasmid
    #37758
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    pLar_rpoS_gfp
    pLtet_dsrA_pLac_araC
    Use
    Synthetic Biology; E. coli
    Tags
    gfp and none
    Expression
    Mutation
    Promoter
    Ptet and pLac and pLar
    Available since
    Aug. 14, 2012
    Availability
    Academic Institutions and Nonprofits only

  40. pHL 279

    Plasmid
    #37757
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    ptet_ompC_gfp
    pLac_st3 TetR_mCherry
    Use
    Synthetic Biology; E. coli
    Tags
    gfp and mCherry
    Expression
    Mutation
    Promoter
    pLac and ptet
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 4401 - 4450 of 6271 results