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MTK1_020

Plasmid

#123683

Purpose

Encodes ConL7 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_031

Plasmid

#123693

Purpose

Encodes ConL2 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_032

Plasmid

#123694

Purpose

Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_033

Plasmid

#123695

Purpose

Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_034

Plasmid

#123696

Purpose

Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_003

Plasmid

#123698

Purpose

Encodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pUAS (mini SV40)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_012

Plasmid

#123675

Purpose

Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK1_015

Plasmid

#123678

Purpose

Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4-LoxP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.178

Plasmid

#119645

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS3 Down Flank

Use

Synthetic Biology

Mutation

4 nucleotide insertion between nt 65 and 66 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.175

Plasmid

#119643

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Up Flank

Use

Synthetic Biology

Mutation

bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.171

Plasmid

#119639

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Up Flank

Use

Synthetic Biology

Mutation

A691T in NS4 Up Flank

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT/Acrosin-GFP-GPI

Plasmid

#122141

Purpose

For making acrosin-GFP-GPI RNA

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Insert

acrosin-GFP-GPI

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7R8

Plasmid

#111041

Purpose

Plasmid expresses the talin2 rod domains R7 and R8

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7

Plasmid

#111039

Purpose

Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Promoter

CMV Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1B01

Plasmid

#71089

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Clk (Clk Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-x16

Plasmid

#71505

Purpose

flySTARR-seq screening vector with x16 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2C10

Plasmid

#70927

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

okr (okr Fly)

Use

Gateway entry vector

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-RpS12-Gateway

Plasmid

#71508

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge