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pMK-mPrrn (5-fold decrease transcription)

Plasmid

#123478

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

mPrrn (5-fold decrease transcription)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-PatpB (PEP functional NEP disabled)

Plasmid

#123469

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PatpB (PEP functional NEP disabled)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-PatpB (PEP and NEP functional)

Plasmid

#123468

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PatpB (PEP and NEP functional)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-PatpB (NEP functional PEP disabled)

Plasmid

#123466

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PatpB (NEP functional PEP disabled)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCF738

Plasmid

#122032

Purpose

EF1a-WNV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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Pmo_1188_TNXB/p plate_10

Plasmid

#107643

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

1188_TNXB/p plate_10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-PqrA

Plasmid

#51195

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

PqrA

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Lifeact-mCherry

Plasmid

#193300

Purpose

mammalian transient expression - expression of Lifeact-mCherry

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange2-Peroxisomes-2

Plasmid

#54596

Purpose

Localization: Peroxisomes, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

mOrange2-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pFUW H2B-mTagBFP2-P2A-PuroR

Plasmid

#239552

Purpose

Expression and nuclear localization of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin2

Plasmid

#239018

Purpose

Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2-P2A-PuroR

Plasmid

#239565

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSAR-MT-APC

Plasmid

#16487

Depositor

Bert Vogelstein

Article

Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

Insert

APC (APC Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only