We narrowed to 5,347 results for: cycs

1. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_TIAL1

   Plasmid

   #106092

   Purpose

   bacterial protein expression of TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   TIAL1 (TIAL1 Human)
   

   Use

   Tags

   6xHis-thrombin and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBluescript goosecoid

   Plasmid

   #16935

   Depositor

   John Postlethwait

   Article

   Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

   Insert

   goosecoid (gsc Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCB6HindIII-rat FcRn

   Plasmid

   #121671

   Purpose

   Expresses rat neonatal Fc receptor in mammalian cells

   Depositor

   Pamela Bjorkman

   Article

   Tesar et al Traffic. 2006 Sep;7(9):1127-42.

   Insert

   rat neonatal Fc receptor (Fcgrt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXGFPC-5 Pxyl-dcas9 (Spa)

   Plasmid

   #133318

   Purpose

   expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pasteurianus)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPEE17

   Plasmid

   #128361

   Purpose

   Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA2867

   Plasmid

   #25439

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   mCherry-miR155-lacZalpha (Mir155hg Discosoma-mouse-E. coli, Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-PHYT

   Plasmid

   #53300

   Purpose

   Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pADH139

   Plasmid

   #90987

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C, mal LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSV-p65S276A

    Plasmid

    #106455

    Purpose

    NF-kB p65, kinase site 276 mutated

    Depositor

    Georg Weber

    Article

    He et al Eur J Biochem. 2003 May . 270(10):2174-8

    Insert

    NF-kB p65, kinase site 276 mutated (RELA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    kinase site inactivated

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-MKRN1_H307E

    Plasmid

    #78756

    Purpose

    To overexpress MKRN1_H307E in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H307E

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR33

    Plasmid

    #69254

    Purpose

    phsp16.2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.2

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABM-D4-miR30-L1221

    Plasmid

    #132933

    Purpose

    Lentivector for luciferase (control) knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAPM-D4-miR30-TRIM5

    Plasmid

    #132932

    Purpose

    Lentivector for TRIM5 knockdown and puromycin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    TRIM5 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3

    Plasmid

    #106115

    Purpose

    Lentiviral expression of PRKRA delta3

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK and myc

    Expression

    Mutation

    Truncation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

    Plasmid

    #106116

    Purpose

    Lentiviral expression of PRKRA delta 12

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Truncation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK357

    Plasmid

    #71568

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge