We narrowed to 16,997 results for: igh

1. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ReNL_pRSETb

   Plasmid

   #89536

   Purpose

   Red color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OeNL/pcDNA3

   Plasmid

   #85202

   Purpose

   Orange color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Orange enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSLC-284

   Plasmid

   #124294

   Purpose

   A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

   Depositor

   Swaine Chen

   Article

   Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

   Insert

   vsfGFP-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA FLAG TORC2

   Plasmid

   #22975

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

   Insert

   TORC2 (CRTC2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-Citroff1

   Plasmid

   #134301

   Purpose

   Bacterial expression of inverse-response citrate sensor with high binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1
   

   Use

   Tags

   6-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YeNL_pRSETb

   Plasmid

   #89520

   Purpose

   Yellow color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-FB-orig BA

    Plasmid

    #13420

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    orig BA
    

    Use

    Tags

    Gal11P

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL2B -938/+64

    Plasmid

    #24943

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-iGlu3Fast

    Plasmid

    #226500

    Purpose

    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 S72T
    

    Use

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    S72T

    Promoter

    CMV

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCalpha EV (PKA catalytic subunit Calpha)

    Plasmid

    #15310

    Depositor

    Stanley McKnight

    Article

    Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

    Insert

    PRKACA (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Tet

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ReNL-H2B_pcDNA3

    Plasmid

    #89534

    Purpose

    Red color variant of bright luminescent protein, fused to histone 2B

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cox8x2-CeNL_pcDNA3

    Plasmid

    #89513

    Purpose

    Cyan color variant of bright luminescent protein, targeted to the Mitochondria

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    CoxVIII X2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cox8x2-ReNL_pcDNA3

    Plasmid

    #89533

    Purpose

    Red color variant of bright luminescent protein, targeted to the Mitochondria

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    CoxVIII X2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCbeta EV (PKA catalytic subunit Cbeta)

    Plasmid

    #15311

    Depositor

    Stanley McKnight

    Article

    Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

    Insert

    PRKACB (Prkacb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only