We narrowed to 7,290 results for: CAD;

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/α-catenin fusion-pcDNA3

Plasmid

#45771

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)

Expression

Mammalian

Mutation

Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1859A

Plasmid

#46239

Purpose

FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1859 changed to Alanine (S1859A)

Promoter

cmv

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
proE-cad178-Luc-mEbox

Plasmid

#42082

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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proE-cad670-Luc-mEbox

Plasmid

#42084

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

Plasmid

#164262

Purpose

Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1

Expression

Bacterial

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-S586A-VLCAD-FLAG

Plasmid

#55824

Purpose

Expresses S586A VLCAD mutant in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Tags

FLAG tag

Expression

Mammalian

Mutation

changed Serine 586 toAlanine

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS xenopus paraxial protocadherin

Plasmid

#24687

Depositor

Edward De Robertis

Article

Kim et al Development. 1998 Dec . 125(23):4681-90.

Insert

xenopus paraxial protocadherin (pcdh8.2.L Frog)

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-A3 [N144/5R]

Plasmid

#222154

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-A3 (Mouse). Derived from hybridoma N144/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Gamma-protocadherin-A3 (Mus musculus) recombinant mouse monoclonal antibody. (Pcdhga3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tet2puro-gal4DBD-HIFCAD

Plasmid

#207173

Purpose

Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD

Use

Lentiviral; Dox inducible

Tags

2x Myc

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Gamma-protocadherin-A3 scFv [N144/17]

Plasmid

#206739

Purpose

Mammalian Expression of Gamma-protocadherin-A3 scFV. Derived from hybridoma N144/17 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Gamma-protocadherin-A3 (Mus musculus) recombinant scFV (Pcdhga3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-B2 [N148/30]

Plasmid

#190299

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-B2 (Mouse). Derived from hybridoma N148/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Gamma-protocadherin-B2 (Mus musculus) recombinant Mouse monoclonal antibody (Pcdhgb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA1-gltA2-GapAP1

Plasmid

#87150

Purpose

gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

gltA1-gltA2-gapAP1 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prps3

Availability

Academic Institutions and Nonprofits only

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