We narrowed to 26,916 results for: Cat

1. SpyCatcher002-oHex

   Plasmid

   #124670

   Purpose

   Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHex
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpyCatcher002-oPent

   Plasmid

   #124664

   Purpose

   Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oPent
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP783_SpyCatcher-DNMT3A-CO

   Plasmid

   #211776

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavudin-DNMT3A
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpyCatcher002-oHept

   Plasmid

   #124671

   Purpose

   Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHept
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_6xHis-PARP1delCAT

   Plasmid

   #173942

   Purpose

   Bacterial expression of PARP1 lacking CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delCAT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 661-1014

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 4 gRNA concatemer

   Plasmid

   #84881

   Purpose

   Empty concatmer vector in which 4 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP784_SnoopCatcher-DNMT3A-CO

   Plasmid

   #211777

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Spy-EZH2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. InterCatch-1xL

   Plasmid

   #186907

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-CathespinB-6

    Plasmid

    #55007

    Purpose

    Localization: Lysosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. 2 gRNA concatmer

    Plasmid

    #84879

    Purpose

    Empty concatmer vector in which 2 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pDEST14-SpyCatcher EQ

    Plasmid

    #35045

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher EQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. LLP787_SnoopCatcher-EZH2-FL-CO

    Plasmid

    #211780

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP780_SpyCatcher-KRAB-CO

    Plasmid

    #211773

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-KRAB
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-SpyCatcher002 EQ

    Plasmid

    #102830

    Purpose

    Bacterial expression of unreactive version of SpyCatcher002

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002 EQ
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    E77Q mutation prevents isopeptide bond formation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3 gRNA concatemer

    Plasmid

    #84880

    Purpose

    Empty concatmer vector in which 3 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pET26b(+)-ATI_QconCAT_1

    Plasmid

    #163957

    Purpose

    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

    Depositor

    Stefan Tenzer

    Article

    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

    Insert

    ATI QconCAT
    

    Use

    Tags

    polyhistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

    Plasmid

    #116963

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dendra2-CathespinB-6

    Plasmid

    #57703

    Purpose

    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits