We narrowed to 1,279 results for: MS2

1. pFH2.19_mCherry-MS2x2

   Plasmid

   #205534

   Purpose

   Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmKate2-MS2coatProtein

   Plasmid

   #103833

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   mKate2-MS2 coat protein (cp )
   

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_mCherry_2A_HA_2xMS2-VP64

   Plasmid

   #68419

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   CMV

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-3' MS2-CircRNA

   Plasmid

   #213751

   Purpose

   For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUBC_V5_MS2-mVenus

   Plasmid

   #107606

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   MS2-mVenus
   

   Use

   Synthetic Biology

   Tags

   V5 and mVenus

   Mutation

   MS2: V75E/A81G

   Promoter

   human UBC

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.20_mCherry-MS2x4

   Plasmid

   #205535

   Purpose

   Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC051-HisMS2_PLP_pac_LC

   Plasmid

   #128234

   Purpose

   Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET16b-Mms2

   Plasmid

   #18893

   Depositor

   Cecile Pickart

   Article

   Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

   Insert

   Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
   

   Tags

   HIS10

   Expression

   Bacterial

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pFH2.21_mCherry-MS2x6

   Plasmid

   #205536

   Purpose

   Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-MS2_V

    Plasmid

    #147737

    Purpose

    Insect Expression vector

    Depositor

    Elisa Izaurralde

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available Since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pKLV2-EF1aMS2p65HSF1hyg-W

    Plasmid

    #112920

    Purpose

    Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    MS2p65HSF1-T2Ahyg
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT-TODestFLAGHA_RBPMS2_HindIII

    Plasmid

    #65666

    Purpose

    FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MS2_SC2_S_B.1.640

    Plasmid

    #180228

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    B.1.640 (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. MS2_SC2_S_C.1.2

    Plasmid

    #177367

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    C1.2 (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 20, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pFRT-TODestGFP_RBPMS2

    Plasmid

    #65747

    Purpose

    expresses GFP-tagged RBPMS2 in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S13N mutation in RBPMS2

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TODestMYC_RBPMS2

    Plasmid

    #65668

    Purpose

    MYC expression vector with RBPMS2

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    introduced HindIII site to exchange FLAG-HA tag w…

    Promoter

    CMV, 2x Tet

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR™221_RBPMS2

    Plasmid

    #59389

    Purpose

    pDONR vector with RBPMS2

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Promoter

    T7

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS22H-IRE

    Plasmid

    #133905

    Purpose

    Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRE (Ftl1 Rat)
    

    Expression

    Yeast

    Promoter

    Pol III

    Availability

    Academic Institutions and Nonprofits only

    

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20. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

    Plasmid

    #61762

    Purpose

    Dual Color betaGlobin reporter to study splicing kinetics in vivo

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    b-Globin
    

    Tags

    24 X MS2 hairpin cassette in 3'UTR of b-Glob…

    Expression

    Mammalian

    Promoter

    Dox inducible Tet-ON promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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