We narrowed to 4,658 results for: crispr c plasmids

1. qTAG-C-mStayGold-EFS-Puro-PLK4

   Plasmid

   #227311

   Purpose

   Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-C-Solo-mStayGold-COX8A

   Plasmid

   #227309

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mStayGold-Puro-CLTC

   Plasmid

   #227314

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-Solo-mStayGold-CLTC

   Plasmid

   #227313

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-C-mStayGold-EFS-Blast-ARL13B

   Plasmid

   #227278

   Purpose

   Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

   Plasmid

   #227333

   Purpose

   MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-C-mStayGold-EFS-Puro-CEP192

   Plasmid

   #227290

   Purpose

   Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-NG-CBE C

   Plasmid

   #227422

   Purpose

   expresses the C-terminal of AAV-NG-CBE

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   AAV-NG-CBE C-terminal
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GEARBOCS-SPARCL1-C-mCherryTag

    Plasmid

    #218183

    Purpose

    To tag Hevin with mCherry at its C-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eIF3k sgRNA-2 PX459 plasmid

    Plasmid

    #192256

    Purpose

    expressing sgRNA-2 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eIF3k sgRNA-1 PX459 plasmid

    Plasmid

    #192255

    Purpose

    expressing sgRNA-1 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eIF3f sgRNA-1 PX459 plasmid

    Plasmid

    #192253

    Purpose

    expressing sgRNA-1 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eIF3f sgRNA-2 PX459 plasmid

    Plasmid

    #192254

    Purpose

    expressing sgRNA-2 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eIF3b sgRNA-2 PX459 plasmid

    Plasmid

    #192250

    Purpose

    expressing sgRNA-2 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eIF3b sgRNA-1 PX459 plasmid

    Plasmid

    #192249

    Purpose

    expressing sgRNA-1 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

19. SKOR1-gRNA-C-del

    Plasmid

    #131332

    Purpose

    gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    SKOR1-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TS-PE2-C-WPRE

    Plasmid

    #176650

    Purpose

    Expresses C-terminus of PE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    C-terminus of PE2
    Artificial SA
    WPRE
    bGH poly(A)
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge