We narrowed to 4,671 results for: ado

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pTwist-WIV1-CoV Δ18

Plasmid

#164439

Purpose

Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike truncated to remove 18 amino acids

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBK092

Plasmid

#183146

Purpose

pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_ERG_P2A_Hygro_Barcode

Plasmid

#120434

Purpose

Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ERG (ERG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

Plasmid

#140163

Purpose

Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2

Plasmid

#164435

Purpose

Encodes SARS-CoV-2 Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2

Plasmid

#164433

Purpose

Encodes SARS-CoV-2 Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-A2AR

Plasmid

#106066

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-A2AR (ADORA2A Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-1.7kbfabp6

Plasmid

#159087

Purpose

p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Insert

1.7kb fabp6 promoter (fabp6 Zebrafish)

Use

Promoter fragment 5' entry vector for tol2 c…

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Flag-LplA

Plasmid

#232118

Purpose

For bacterial expression of Flag-tagged E. coli LplA.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

lplA (lplA Escherichia coli (strain K12))

Tags

Flag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_LHX3_P2A_Hygro_Barcode

Plasmid

#120456

Purpose

Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LHX3 (LHX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXP1_P2A_Hygro_Barcode

Plasmid

#120441

Purpose

Barcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXP1 (FOXP1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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