We narrowed to 2,202 results for: K-Ras protein

1. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75244

   Purpose

   CRISPR/Cas9 plasmid against human Helios

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75238

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75239

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV40-EF1a-hBCL10(1-114)-SO

   Plasmid

   #75257

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   AS 1-116 (CARD-domain)

   Promoter

   pMSCV-LTRs, EF1a

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-TetR-neoR

   Plasmid

   #153310

   Purpose

   Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Insert

   TetR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

   Plasmid

   #169191

   Purpose

   To express nsp10-nsp16 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

   Insert

   14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBS-864

   Plasmid

   #218018

   Purpose

   GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   GST-tag
   

   Use

   Synthetic Biology

   Tags

   TEV protease cleavage site

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBS-848

   Plasmid

   #218003

   Purpose

   GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   GST-tag
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1382

    Plasmid

    #29182

    Purpose

    Expression of the eGFP reporter gene was detected (positive) in the brain, but not detected (negative) in the eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple183 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1385

    Plasmid

    #29184

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple186 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS1383

    Plasmid

    #29183

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple184 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. SARS CoV-2 RBD (bio-His)

    Plasmid

    #166857

    Purpose

    Human expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.

    Depositor

    Evelien Bunnik

    Article

    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

    Insert

    SARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    6xHis and Biotinylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV-MAT2B

    Plasmid

    #34795

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    methionine adenosyltransferase II, beta (MAT2B Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. D9_Ld_190920

    Plasmid

    #135825

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Use

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-LS

    Plasmid

    #205438

    Purpose

    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Inserts

    Gaussia luciferase gene
    spectinomycin resistance gene
    

    Use

    Cre/Lox and Luciferase; Chlamydomonas expression

    Tags

    Expression

    Mutation

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX307_CR-PDHA2S293A

    Plasmid

    #115204

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307_CR-PDHA2

    Plasmid

    #115199

    Purpose

    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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