We narrowed to 2,328 results for: dap

1. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

6. T-B18

   Bacterial Strain

   #159446

   Purpose

   E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

   Depositor

   Eleftherios Terry Papoutsakis, Maciek R. Antoniewicz

   Article

   Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

   Bacterial Resistance

   Ampicillin and kanamycin

   Available since

   Aug. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

7. Human Kinase Domain Constructs for Automated Bacterial Expression

   Plasmid Kit

   #1000000094

   Purpose

   68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBP-P_phlF-PPhlF

   Plasmid

   #204075

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   phlF repressor and PPhlF DAPG-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

   Plasmid

   #204080

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mr-GST full length

    Plasmid

    #112982

    Purpose

    For protein expression and purification of full-length Drosophila mr (APC2)

    Depositor

    Terry Orr-Weaver

    Insert

    mr (APC2) (mr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. hPLD3-sgRNA-Cas9_mcherry

    Plasmid

    #199342

    Purpose

    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hPLD4-sgRNA-Cas9_mcherry

    Plasmid

    #199343

    Purpose

    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    sgRNA: accagtagtatgaagccacg

    Promoter

    human U6

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTHAP12-donor

    Plasmid

    #176348

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    THAP12 (THAP12 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mr-GST N-term fusion

    Plasmid

    #112983

    Purpose

    For protein expression and purification of N-terminus of Drosophila mr (APC2)

    Depositor

    Terry Orr-Weaver

    Insert

    mr (APC2) (mr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-term only

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AbVec_IGHC

    Plasmid

    #183701

    Purpose

    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGHG1 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AbVec_IGLC

    Plasmid

    #183703

    Purpose

    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGLC2 (IGLC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-ABE7.10-P2A-Puro

    Plasmid

    #112674

    Purpose

    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

    Depositor

    Lukas Dow

    Insert

    ABE7.10
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge