We narrowed to 7,636 results for: donor

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AICSDP-29: LMNB1-mTagRFP-T

Plasmid

#114403

Purpose

Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1

Depositor

Allen Institute for Cell Science

Insert

LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)

Use

CRISPR; Donor template

Tags

mTagRFPT-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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OPTN_Halo_C_allele

Plasmid

#178144

Purpose

Donor vector for endogenous tagging of human OPTN at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (OPTN Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ANG_Halo_C_allele

Plasmid

#178115

Purpose

Donor vector for endogenous tagging of human ANG at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ANG Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PSEN2_Halo_C_allele

Plasmid

#178133

Purpose

Donor vector for endogenous tagging of human PSEN2 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PSEN2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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KCND3_Halo_C_allele

Plasmid

#178162

Purpose

Donor vector for endogenous tagging of human KCND3 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KCND3 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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DCTN1_Halo_C_allele

Plasmid

#178156

Purpose

Donor vector for endogenous tagging of human DCTN1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DCTN1 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-64: FUS-mEGFP

Plasmid

#114410

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS

Depositor

Allen Institute for Cell Science

Insert

FUS Homology Arms with linker-mEGFP (FUS Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBFC1207

Plasmid

#186694

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AICSDP-64: FUS-mEGFP

Plasmid

#114410

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS

Depositor

Allen Institute for Cell Science

Insert

FUS Homology Arms with linker-mEGFP (FUS Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-39:RAB5A-mEGFP

Plasmid

#107579

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

Depositor

Allen Institute for Cell Science

Insert

RAB5A Homology Arms with mEGFP-linker (RAB5A Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 9, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pYS17

Plasmid

#202083

Purpose

Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT PuroR-T2A-mNG Minicircle Parent

Use

CRISPR

Tags

mNeonGreen

Promoter

None

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-sfGFP-3xP3-RFP

Plasmid

#127566

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Expression

Insect

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-60: SOX2-mEGFP

Plasmid

#124606

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

Depositor

Allen Institute for Cell Science

Insert

SOX2 Homology Arms with linker-mEGFP (SOX2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

May 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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