We narrowed to 7,417 results for: erf

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA

Plasmid

#108844

Purpose

Insert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 BTF3

Plasmid

#67059

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BTF3 (BTF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
NET4 pEGFP-N2 (1201)

Plasmid

#62041

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

Net4 (TMEM53 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133347

Purpose

expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA and sgRNA_blaA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

A435S

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK-Venus-TmiR-G13

Plasmid

#25741

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 BMPR2

Plasmid

#67127

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR2 (BMPR2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pVRa30_83

Plasmid

#49686

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF30_83

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12-S35

Plasmid

#39511

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-G37

Plasmid

#39512

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and E37G

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-C40

Plasmid

#39515

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-Fgß-FIVAR-ELP-HIS-ybbR

Plasmid

#169136

Purpose

E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

FIVAR (found in various architectures)

Tags

3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-675-GB1_AD

Plasmid

#148504

Purpose

Bacterial Expression of DmeIF4G_578-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_601-634-GB1_AD

Plasmid

#148505

Purpose

Bacterial Expression of DmeIF4G_601-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_601-675-GB1_AD

Plasmid

#148507

Purpose

Bacterial Expression of DmeIF4G_601-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_617-634-GB1_AD

Plasmid

#148508

Purpose

Bacterial Expression of DmeIF4G_617-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_617-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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