We narrowed to 24,695 results for: lars

1. pcDNA 3.3 HA-TSPAN12^11-LEL

   Plasmid

   #115785

   Purpose

   Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   large extracellular loop of TSPAN12 replaced with…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FKBP-GTSE1(638-720)-GFP

   Plasmid

   #100735

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   C-terminus only

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-delta/gamma2SIL

   Plasmid

   #119731

   Purpose

   GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRD/GABRG2 (Gabrg2, Gabrd Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1585588

   Plasmid

   #62620

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   dihydroflavonol 4-reductase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTFP1-Gamma-Tubulin-17

   Plasmid

   #55485

   Purpose

   Localization: Centrosomes, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Sema6b(S)-Fc-His

   Plasmid

   #72166

   Purpose

   Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6b (Sema6b Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTFP1-ELP-1-25

   Plasmid

   #55478

   Purpose

   Localization: Golgi/ER, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   ELP (KDELR2 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. GFP-PODXL FBM*

   Plasmid

   #195220

   Purpose

   Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PODXL (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H477/R479/S481>AAA

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-PODXL S481A

   Plasmid

   #195223

   Purpose

   Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PODXL (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S481A

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid

    #213417

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid

    #213414

    Purpose

    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcS-RDG-C1C2

    Plasmid

    #200163

    Purpose

    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

    Depositor

    Masako Harada

    Article

    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

    Inserts

    RDG-C1C2
    C1C2 domain of lactadherin
    

    Use

    Tags

    HA and HIS

    Expression

    Mammalian

    Mutation

    sequence adopted from doi: 10.1371/journal.pone.0…

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMK ENTR 2 hU6 ACTB sgRNA

    Plasmid

    #206270

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    ACTB sgRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 2 hU6 HEK3 PegRNA

    Plasmid

    #206277

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HEK3 PegRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMK ENTR 3 polH aeBlue polH VSV-G

    Plasmid

    #206278

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    aeBlue, VSV-G
    

    Use

    Multimate/gateway entr 3

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T3-GST-PolB(K72A)

    Plasmid

    #177132

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Flag-PolB(T304I)-Puro

    Plasmid

    #177143

    Purpose

    Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX4T3-GST-PolB(K206A/K244A)

    Plasmid

    #177135

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEntr1a-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176134

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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