We narrowed to 10,294 results for: otos
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Plasmid#165439PurposeExpresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.DepositorInsertmEGFP(A206K)-paCaMKII(T286A)
TagsmEGFP(A206K)ExpressionMammalianMutationT286A: Autophosphorylation deficient mutationPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Aqp4-eGFP
Plasmid#176747PurposeExpresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytesDepositorInsertAqp4-eGFP
UseAAVTagsGFPExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-osTIR1-IRES-GFP
Plasmid#110661PurposeLentiviral vector for stable expression of osTIR1 and selection with GFPDepositorInsertosTIR1
UseLentiviralPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorInsertSaCas9 (NEWENTRY Staphylococcus aureus)
Tags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCAGAvailable SinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorInsertDDdCas9GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII-WPRE3
Plasmid#165429PurposeExpresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.DepositorInsertFHS-paCaMKII
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianPromoterCaMKII0.4Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-Stargazin-mEGFP-iLID
Plasmid#178529PurposeExpresses Stargazin-mEGFP-iLID in mammalian cellsDepositorInsertStargazin-mEGFP-iLID
ExpressionMammalianPromoterCAGAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTfR-PATagRFP
Plasmid#31946DepositorInsertPATagRFP
TagsTfRExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN1-CMV-sDarken
Plasmid#184799PurposeSerotonin Sensor sDarken under the control of a CMV Promoter.DepositorInsertsDarken
ExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-NaChBac
Plasmid#125580PurposeCre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.DepositorInsertNaChBac
UseCre/LoxTagsFLAGExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
ExpressionMammalianPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-ABKAR
Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorInsertAMPK Biosensor
TagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-PATagRFP
Plasmid#31945DepositorInsertPATagRFP
TagsHisExpressionBacterialMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoteraraBAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVSVG-PATagRFP
Plasmid#31947DepositorInsertPATagRFP
TagsVSVGExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-172)
Plasmid#37309DepositorAvailable SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only