Skip to main content
Addgene
Showing: 1801 - 1820 of 23719 results

  1. pSC43

    Plasmid
    #104817
    Purpose
    CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.04g057400
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    104817-plasmid-map-sequence-id-198651

    Enlarge

  2. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

    Plasmid
    #115139
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3.1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115139-plasmid-map-sequence-id-220497

    Enlarge

  3. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

    Enlarge

  6. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid
    #115140
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS
    Depositor
    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115140-plasmid-map-sequence-id-220495

    Enlarge

  7. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid
    #115138
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
    Depositor
    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115138-plasmid-map-sequence-id-220494

    Enlarge

  8. pSC50

    Plasmid
    #104824
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.08g081600, Glyma.05g126600
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104824-plasmid-map-sequence-id-203137

    Enlarge

  10. pPgiCas13b-gRNA-1

    Plasmid
    #184834
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184834-plasmid-map-sequence-id-377030

    Enlarge

  11. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

    Enlarge

  12. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

    Enlarge

  16. pSC51

    Plasmid
    #104825
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.12g075700, Glyma.11g145900
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    104825-plasmid-map-sequence-id-203148

    Enlarge

  18. IL1RN gRNA4

    Plasmid
    #113133
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, Synthetic Biology, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113133-plasmid-map-sequence-id-226956

    Enlarge

Showing: 1801 - 1820 of 23719 results