We narrowed to 13,845 results for: cas9

1. pDonor-Prf1-ires-mCherry

   Plasmid

   #209073

   Purpose

   Targeting vector for the mouse Prf1 locus to correct prf1 gene

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor-Prf1-mock-ires-mCherry

   Plasmid

   #209074

   Purpose

   Targeting vector for the mouse Prf1 locus to correct prf1 gene

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS12<ActB.mus_2A-mCh_KI-HMEJ

   Plasmid

   #207407

   Purpose

   Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Actb (Actb Mouse)
   

   Use

   CRISPR and Mouse Targeting; Hmej knockin donor

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Chr1q_Cassette-Proximal_gRNA

   Plasmid

   #195127

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

   Plasmid

   #160560

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M3_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

   Plasmid

   #160558

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

   Plasmid

   #160559

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M2_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

   Plasmid

   #160625

   Purpose

   GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26-1gRNA-DFR 2.1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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