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Addgene

We narrowed to 18,818 results for: RAN-1

Showing: 4551 - 4600 of 18818 results
  1. pCL12-decoder

    Plasmid
    #159447
    Purpose
    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Insert
    PlexAtet-Citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pR I-Ab beta Derp1-117

    Plasmid
    #113639
    Purpose
    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide
    Depositor
    Insert
    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    Use
    Tags
    6x His tag, fused to Der p 1 (117-127) peptide vi…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCAROTENE5X

    Plasmid
    #208816
    Purpose
    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell
    Depositor
    Insert
    Five copies of JUB1 binding site within the synthetic promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    JUB1 TF-responsive promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pRPa-iSL(MCS1+2)

    Plasmid
    #69244
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pSP571

    Plasmid
    #139498
    Purpose
    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
    Depositor
    Insert
    pGPD Cas9 / sgRNA (STL162)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pGPD
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pJUB2x

    Plasmid
    #208814
    Purpose
    Encodes GFP under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell
    Depositor
    Insert
    Two copies of JUB1 binding site within the synthetic promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    JUB1 TF-responsive promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pZA40

    Plasmid
    #158518
    Purpose
    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-6xHA. Km plant selection marker.
    Depositor
    Insert
    ZAR1-6xHA
    Use
    Tags
    6xHA
    Expression
    Plant
    Mutation
    L17E, D481V
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pZA32

    Plasmid
    #158510
    Purpose
    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-4xMyc. Km plant selection marker.
    Depositor
    Insert
    ZAR1-4xMyc
    Use
    Tags
    4xMyc
    Expression
    Plant
    Mutation
    L17E, D481V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pZA48

    Plasmid
    #158526
    Purpose
    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.
    Depositor
    Insert
    ZAR1-eGFP
    Use
    Tags
    eGFP
    Expression
    Plant
    Mutation
    L17E, D481V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pYFP-x-10

    Plasmid
    #140143
    Purpose
    Evaluating the impact of high sensor concentration on information transmission of a genetic sensor.
    Depositor
    Insert
    lacY (lacY )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D37, I38, and N39 are changed to nonsense (stop) …
    Promoter
    none
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pYFP-x-.008

    Plasmid
    #140141
    Purpose
    Evaluating the impact of low sensor concentration on information transmission of a genetic sensor.
    Depositor
    Insert
    lacY (lacY )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D37, I38, and N39 are changed to nonsense (stop) …
    Promoter
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1

    Plasmid
    #116974
    Purpose
    Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout
    Depositor
    Insert
    Candidatus Accumulibacter sp. BA-91 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC

    Plasmid
    #116968
    Purpose
    Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout
    Depositor
    Insert
    Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1

    Plasmid
    #116967
    Purpose
    Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout
    Depositor
    Insert
    Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pRP-c6MYCn

    Plasmid
    #69243
    Purpose
    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  16. pRPa-iSL(MCS3+4)

    Plasmid
    #69246
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  17. p5154

    Plasmid
    #175390
    Purpose
    High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. p5155

    Plasmid
    #175391
    Purpose
    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Promoter
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. DPAGT1 (DPAGT1A-c005)

    Plasmid
    #98228
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Insert
    DPAGT1A (DPAGT1 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam00953:Glycos_transf_4 98-275
    Promoter
    polyhedrin
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  22. pCR4_miniFXN_8

    Plasmid
    #234361
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 4551 - 4600 of 18818 results