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Showing: 4551 - 4600 of 5794 results

  3. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid
    #113956
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113956-plasmid-map-sequence-id-215284

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  11. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid
    #113952
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S and S212N su…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113952-plasmid-map-sequence-id-215166

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  15. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Roger Tsien, Erik Rodriguez
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  22. pcDNA-Prom1 D-5

    Plasmid
    #184017
    Purpose
    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.
    Depositor
    Peter Stoilov
    Article
    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d
    Insert
    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Deletion of exon 19a (amino acids 696-701), and d…
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184017-plasmid-map-sequence-id-362468

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  25. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Article
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Expression
    Mutation
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    129531-plasmid-map-sequence-id-255632

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  27. TFORF3454

    Plasmid
    #144930
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF653 (ZNF653 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144930-plasmid-map-sequence-id-404500

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  32. pAAV-DYRK2-2A-GFP

    Plasmid
    #118284
    Purpose
    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    Use
    AAV
    Tags
    2A peptide and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118284-plasmid-map-sequence-id-229473

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  36. pCEP4-CH115gp160-QES.c14-754*

    Plasmid
    #123282
    Purpose
    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (CH115) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    123282-plasmid-map-sequence-id-235248

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  39. pCEP4-HA-Flag-natT1R2 a22-end_S212N

    Plasmid
    #113951
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution
    Promoter
    CMV
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113951-plasmid-map-sequence-id-215164

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  48. Ntng2.a-Fc-His

    Plasmid
    #72120
    Purpose
    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng2.a (Ntng2 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72120-plasmid-map-sequence-id-131306

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  50. Ntng1.b-Fc-His

    Plasmid
    #72111
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.b (Ntng1 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72111-plasmid-map-sequence-id-139922

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Showing: 4551 - 4600 of 5794 results