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Showing: 1821 - 1840 of 12332 results

  6. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid
    #225438
    Purpose
    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.
    Depositor
    Diego Orzaez
    Insert
    CBS-FRT-OCS-BeYDVRepRepA-tNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225438-plasmid-map-sequence-id-447740

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  7. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

    Plasmid
    #225436
    Purpose
    BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.
    Depositor
    Diego Orzaez
    Insert
    BeYDV geminino 1.0
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225436-plasmid-map-sequence-id-447735

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  8. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

    Plasmid
    #225430
    Purpose
    OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter
    Depositor
    Diego Orzaez
    Insert
    miniDFR-FRT-OCSterm-FRT-miniDFR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225430-plasmid-map-sequence-id-447730

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  9. pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

    Plasmid
    #225431
    Purpose
    OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter
    Depositor
    Diego Orzaez
    Insert
    miniDFR-att-OCSterm-att-miniDFR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225431-plasmid-map-sequence-id-447728

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  10. pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

    Plasmid
    #225439
    Purpose
    Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.
    Depositor
    Diego Orzaez
    Insert
    HygR-MAR10-gemiGFP-MAR10
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225439-plasmid-map-sequence-id-447723

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  12. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  13. pOET5 GII.4 Sydney noro-VLP

    Plasmid
    #218092
    Purpose
    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).
    Depositor
    Minna Hankaniemi
    Insert
    noro-VLP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218092-plasmid-map-sequence-id-433318

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  15. p663-UBC-miniTurbo-V5-POMK_IDG-K

    Plasmid
    #189893
    Purpose
    Gateway destination clone of POMK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    POMK (POMK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    189893-plasmid-map-sequence-id-381802

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  16. p667-UBC-SBK3-V5-miniTurbo_IDG-K

    Plasmid
    #189900
    Purpose
    Gateway destination clone of SBK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    SBK3 (SBK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    F348S
    Promoter
    Ubiquitin
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    189900-plasmid-map-sequence-id-373998

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  17. p667-UBC-PXK-V5-miniTurbo_IDG-K

    Plasmid
    #189898
    Purpose
    Gateway destination clone of PXK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PXK (PXK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    189898-plasmid-map-sequence-id-374771

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  18. p663-UBC-miniTurbo-V5-STK31_IDG-K

    Plasmid
    #189880
    Purpose
    Gateway destination clone of STK31 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    STK31 (STK31 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189880-plasmid-map-sequence-id-374486

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  19. p663-UBC-miniTurbo-V5-WEE2_IDG-K

    Plasmid
    #189895
    Purpose
    Gateway destination clone of WEE2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    WEE2 (WEE2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189895-plasmid-map-sequence-id-374472

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  20. p663-UBC-miniTurbo-V5-PAK5_IDG-K

    Plasmid
    #189896
    Purpose
    Gateway destination clone of PAK5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PAK5 (PAK5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189896-plasmid-map-sequence-id-374471

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Showing: 1821 - 1840 of 12332 results