We narrowed to 21,301 results for: BASE;

1. pJT78_GalL_nCDA1Δ195-BE3

   Plasmid

   #145044

   Purpose

   Expresses nCDA1Δ195-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ195-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-195AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PKAsub-YPet-Kras

   Plasmid

   #138206

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-Kras
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mCherry-PKCsub-RLuc8N-NES

   Plasmid

   #138213

   Purpose

   Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRE5

   Plasmid

   #62377

   Purpose

   Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p667-UBC-GFP-V5_IDG-K

   Plasmid

   #135242

   Purpose

   Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   GFP-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 H2B-mIFP T2A Mpro (C145A)

   Plasmid

   #163080

   Purpose

   Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Inactive Mpro mutant C145A and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG10-pGlnA-mCherry

   Plasmid

   #102467

   Purpose

   Expresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 122606

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pGlnA

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRHA12

   Plasmid

   #138942

   Purpose

   AND gate with IPTG and aTc as inputs and sfGFP as output

   Depositor

   Guillermo Rodrigo

   Article

   Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

   Insert

   riboregulated sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-V5-dsRed_IDG-K

    Plasmid

    #135262

    Purpose

    Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-dsRed
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRHA40

    Plasmid

    #138941

    Purpose

    XOR gate with IPTG and aTc as inputs and mRFP1 as output

    Depositor

    Guillermo Rodrigo

    Article

    Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

    Insert

    riboregulated mRFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-hcRed-V5_IDG-K

    Plasmid

    #135239

    Purpose

    Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    hcRed-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-V5-eGFP_IDG-K

    Plasmid

    #135265

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG05-pGlnA-deGFP

    Plasmid

    #102462

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGlnA

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC V5 MCS IRES G418

    Plasmid

    #110345

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid

    #110375

    Purpose

    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    turboGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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