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CAG-Flex-RG

Plasmid

#48333

Purpose

Cre-dependant (flex) rabies glycoprotein

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

Rabies Glycoprotein

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Promoter

EF1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP(V5_H2B_APEX2)

Plasmid

#107597

Purpose

Expresses V5_H2B_APEX2 in mammallian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

H2B-APEX2

Tags

H2B and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UbcP

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Mutation

K44A

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Tags

Golgi and ER export signal

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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