We narrowed to 12,296 results for: ache

1. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

   Plasmid

   #118412

   Purpose

   Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

   Depositor

   Nicole Paulk

   Article

   Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

   Insert

   Firefly luciferase
   

   Use

   AAV, Luciferase, and Synthetic Biology

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SHP-2(NC)-SH2

   Plasmid

   #46499

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(NC) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-222

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX SHP-2(C)-SH2

   Plasmid

   #46497

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(C) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 101-222

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-SpCas9

   Plasmid

   #130968

   Purpose

   3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   3xHA-NLS-SpCas9
   

   Use

   CRISPR

   Tags

   3x HA, EGFP, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pαH-S-GSAS-deltaH69/V70

   Plasmid

   #171744

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pαH-S-GSAS-B.1.351.v5

   Plasmid

   #173790

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein South African variant version 5

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pαH-S-GSAS-D614G-N501Y

   Plasmid

   #171748

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pαH-S-GSAS-D614G-E484K

   Plasmid

   #171751

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pαH-S-GSAS-D614G-K417N

   Plasmid

   #171752

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pαH-S-GSAS-D614G-I692V

    Plasmid

    #171746

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-GSAS-D614G-Y453F

    Plasmid

    #171745

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX458-3xHA-dFnCas9

    Plasmid

    #201954

    Purpose

    Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dFnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A and H969A on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-sgBrip1#2/Cre

    Plasmid

    #193204

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgBrip1#2 (Brip1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-sgBrip1#1/Cre

    Plasmid

    #193203

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgBrip1#1 (Brip1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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