165,185 results
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pCJ189
Plasmid#162666PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3A
Plasmid#50857Purposeexpresses constituvely active cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
E326K GBA pcDNA3.1
Plasmid#188581PurposeEctopic expression E326K GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationE326KPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_ATG4B_BamHI_NotI
Plasmid#190862PurposePlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392DepositorInsertATG4B (ATG4B Human)
UseTagsGST, Thrombin Cleavage siteExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike-VLP
Plasmid#173922PurposeExpresses virus-like particles based upon the original SARS-Cov-2DepositorInsertsUseTagsno tags, native proteinExpressionBacterial and MammalianMutationPromoter22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…Available sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
plenti-CAG-IRF4-FLAG-IRES-GFP
Plasmid#107389PurposeLentiviral expression of IRF4-FLAGDepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
