We narrowed to 974 results for: ggct

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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sgAPC(A)

Plasmid

#85557

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
Lenti-sgCrebbp#1/Cre

Plasmid

#193209

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCrebbp#1 (Crebbp Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1E KI

Plasmid

#131481

Purpose

Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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ABL1 gRNA (BRDN0001147582)

Plasmid

#77732

Purpose

3rd generation lentiviral gRNA plasmid targeting human ABL1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ABL1 (ABL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRFP

Plasmid

#125778

Purpose

constitutive expression of a short-hairpin RNA targeting RFP

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRFP

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-HEK3-CTTins-px330-scaffold

Plasmid

#180017

Purpose

Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3a

Plasmid

#180432

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129048

Purpose

inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA8 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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