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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag DLC2

Plasmid

#24269

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
p6609 MSCV-IP N-HAonly PTENP1

Plasmid

#35008

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PTENP1 (PTENP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE- TRE-DEST-CBioTAP

Plasmid

#53569

Purpose

Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-cPla2-C2-EGFP-GH

Plasmid

#187088

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000116711 (PLA2G4A Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBT344_pattB-pCA-GFP-pA-FRT5

Plasmid

#52550

Purpose

for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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ACS2 Truncated

Plasmid

#13748

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 39–682

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pWZL blast HA goosecoid

Plasmid

#14096

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.(cyto).cpSFGFP.HaloTag

Plasmid

#209662

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGSTag BimL

Plasmid

#24251

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (BCL2L11 Human)

Tags

GST

Expression

Bacterial

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pAC-Cyc

Plasmid

#33156

Depositor

Michael Rosbash

Article

Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

Insert

Cyc (cyc Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Tags

mMaple3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-Cre-rc-attP

Plasmid

#51264

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-Cre-rc-attP

Expression

Mammalian

Promoter

CAG

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJK03_Rho_basal_DsRed

Plasmid

#173490

Purpose

Vector backbone used to clone MPRA libraries

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Bovine Rhodopsin promoter driving DsRed fluorescent protein

Expression

Mammalian

Promoter

Bovine Rhodopsin

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEBG-hBEN

Plasmid

#22160

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-GFP-Patronin CC 868–1457

Plasmid

#59053

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-FlagBcl10K31RK63R

Plasmid

#18719

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Lysine 31 to arginine and Lysine 63 to arginine.

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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hsa-miR-100 plix4.03

Plasmid

#121128

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-100 (MIR100 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEBB-GFP-hBEN

Plasmid

#22157

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGST-GstZ1

Plasmid

#163090

Purpose

Expressing mouse Gstz1 in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

GstZ1
GstZ1

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#2

Plasmid

#183120

Purpose

pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EPAS1-2

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBMFB2

Plasmid

#31278

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Bmal1 (Bmal1 Mouse)

Tags

His

Expression

Insect

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-scrCdh2.1 GFP

Plasmid

#209100

Purpose

GFP expressing scramble of shRNA targeting Cdh2

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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