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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG1

Plasmid

#42226

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS:YSOG1

Expression

Mammalian

Available Since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only

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KvAvs3

Plasmid

#188792

Purpose

Avs3 from Klebsiella variicola PF-1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KvAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B3-4

Plasmid

#111474

Purpose

translational release factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AP-p002

Plasmid

#31493

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
BASIC_1_MB1

Plasmid

#68135

Purpose

storage vector for BASIC part: MB1

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

MB1 origin

Expression

Bacterial

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P279L

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-Nb-SpyCatcher

Plasmid

#243752

Purpose

For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Nb-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SNAP

Plasmid

#243741

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SNAP

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SpyTag

Plasmid

#243739

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1

Plasmid

#242837

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-VAV3

Plasmid

#242834

Purpose

Encodes mouse VAV3 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV3 (Vav3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mTFP1-actin

Plasmid

#229970

Purpose

encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mTFP1-actin

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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