We narrowed to 7,717 results for: Ski;

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala271

Plasmid

#158631

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

Q271A please see depositor comments below

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala271 hygro

Plasmid

#158637

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q271A

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

Plasmid

#158638

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q/Q271A

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93,Ala271

Plasmid

#158632

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

E93Q/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148,Ala271

Plasmid

#158633

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

Q148A/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

Plasmid

#158639

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148 hygro

Plasmid

#158636

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93 hygro

Plasmid

#158635

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

Plasmid

#113691

Purpose

U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-EGFP MASTL G44S (siRNA resistant)

Plasmid

#191012

Purpose

Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

G44S

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL E167D (siRNA resistant)

Plasmid

#191013

Purpose

Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

E167D

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MmACE2 (Mouse)

Plasmid

#158087

Purpose

Expresses mouse ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Mouse ACE2 (Ace2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Promoter

none

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MjACE2 (Pangolin)

Plasmid

#158084

Purpose

Expresses pangolin ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Pangolin ACE2 (ACE2 )

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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