We narrowed to 7,645 results for: Ski;

1. pGS_101

   Plasmid

   #160541

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pscALPSpuro-MjACE2 (Pangolin)

   Plasmid

   #158084

   Purpose

   Expresses pangolin ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Pangolin ACE2 (ACE2 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGS_128

   Plasmid

   #160651

   Purpose

   Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e3 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e3 allele

   Promoter

   pZ estradiol inducible promoter

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pscALPSpuro-HsACE2 (human)

   Plasmid

   #158081

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-mCherry-DMDEx23-eGFP

   Plasmid

   #211366

   Purpose

   Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

   Depositor

   Ulrich Lächelt

   Article

   Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

   Insert

   mCherry-DMDEx23-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry interrupted by mdx dystrophin exon 23 bet…

   Promoter

   CAG

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGS_107

   Plasmid

   #160650

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-EGFP MASTL G44S (siRNA resistant)

   Plasmid

   #191012

   Purpose

   Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G44S

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS_129

    Plasmid

    #160652

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Li-Huei Tsai, Susan Lindquist

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    pZ estradiol inducible promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-EMTB-TurboID-V5-puro

    Plasmid

    #190741

    Purpose

    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    EMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

    Plasmid

    #113694

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS D614G soluble Spike

    Plasmid

    #164651

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pscALPSpuro-SsACE2 (Pig)

    Plasmid

    #158085

    Purpose

    Expresses pig ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Pig ACE2 (ACE2 Sus scrofa)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pscALPSpuro-ClACE2 (Dog)

    Plasmid

    #158083

    Purpose

    Expresses dog ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Dog ACE2 (ACE2 )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pscALPSpuro-RnACE2 (Rat)

    Plasmid

    #158086

    Purpose

    Expresses rat ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Rat ACE2 (Ace2 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pscALPSpuro-FcACE2 (Cat)

    Plasmid

    #158082

    Purpose

    Expresses cat ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Cat ACE2 (ACE2 )
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCIDT-attL1-Human ABeta-attR5

    Plasmid

    #160436

    Purpose

    Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQC MCS IRES Puro

    Plasmid

    #110343

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid

    #135421

    Purpose

    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Tags

    GAP43 axon targeting sequence

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge