We narrowed to 3,335 results for: UBC

1. pSUMO1

   Plasmid

   #52258

   Purpose

   Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 )
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12045-pAAV-3xSP10ins-AiE0359h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2045)

   Plasmid

   #163504

   Purpose

   AiE0359h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ cortical interneurons and cerebellar Purkinje cells

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   MinRho

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer10b-HA-KRAS WT CO SR

   Plasmid

   #164926

   Purpose

   Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

   Depositor

   Ji Luo

   Article

   Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

   Insert

   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mutation

   9 Valine codons are optimized and siRNA targetin…

   Promoter

   Ubc promoter

   Available since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInducer10b-HA-KRAS G12V CO SR

   Plasmid

   #164928

   Purpose

   Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

   Depositor

   Ji Luo

   Article

   Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

   Insert

   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mutation

   Glycine 12 changed to valine; 9 Valine codons are…

   Promoter

   Ubc promoter

   Available since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. UbiGate Components (set1)

   Plasmid Kit

   #1000000144

   Purpose

   Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays

   Depositor

   Marco Trujillo

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Luc.Cre empty vector

   Plasmid

   #20905

   Depositor

   Tyler Jacks

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

   Plasmid

   #167832

   Purpose

   Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   HRAS(N17) (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S17N

   Promoter

   Ubc, TRE

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC224

    Plasmid

    #231169

    Purpose

    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC225

    Plasmid

    #231170

    Purpose

    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJC227

    Plasmid

    #231172

    Purpose

    Empty Module C' plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-LucS

    Plasmid

    #22778

    Depositor

    Tyler Jacks

    Insert

    SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTJK368

    Plasmid

    #138006

    Purpose

    BirA* expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    BirA*
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2PWQ

    Plasmid

    #51353

    Depositor

    Cheryl Arrowsmith

    Insert

    2PWQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    April 4, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

17. FUCas9GFP

    Plasmid

    #85555

    Purpose

    Expresses Cas9 with green fluorescent reporter

    Depositor

    Marco Herold

    Insert

    humanised S.pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTJK422

    Plasmid

    #138002

    Purpose

    SIX2 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUCas9Cyan

    Plasmid

    #85554

    Purpose

    Expresses Cas9 with fluorescent Cyan reporter

    Depositor

    Marco Herold

    Insert

    humanised S.pyrogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTJK299

    Plasmid

    #138000

    Purpose

    SIX1 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    SIX1 (SIX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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