We narrowed to 1,247 results for: ncl
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Plasmid#86656PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation2120 base pairs including up to coding nucleotide…PromoterCDK4Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
TagsDHFR and EGFPExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ctcf-Halo-mAID donor
Plasmid#113103PurposeDonor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequenceDepositorInsertc terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)
UseSynthetic BiologyAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPPH-T2A-GFP-shP53
Plasmid#102897PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPPH-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.puro.MCV.ER.RAZ.2
Plasmid#114382PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralExpressionMammalianPromoterMCV NCCR (viral own promoter)Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
ExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only