We narrowed to 18,726 results for: IRE

1. pMM0625

   Plasmid

   #129000

   Purpose

   pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMM0557

   Plasmid

   #128991

   Purpose

   LEU2 integration vector for Zif268DBD-CRY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2-tADH1-URA3-Ura3 5' homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM0558

   Plasmid

   #128992

   Purpose

   LEU2 integration vector for Zif268DBD-CRY2PHR

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2PHR-tADH1-URA3-Ura3 5' homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMM0657

   Plasmid

   #129018

   Purpose

   pRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMM0656

   Plasmid

   #129017

   Purpose

   pTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMM0317

   Plasmid

   #128979

   Purpose

   Encodes Zif268DBD-CRY2 under pscADH1

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM0320

   Plasmid

   #128980

   Purpose

   Encodes Zif268DBD-CRY2PHR under pscADH1

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMM0629

   Plasmid

   #129004

   Purpose

   pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM0521

   Plasmid

   #128984

   Purpose

   Expression plasmid for ZIF268DBD-L3-CRY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   SV40NLS-Zif268DBD-CRY2 (L3) scTRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM0620

    Plasmid

    #128995

    Purpose

    pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM0628

    Plasmid

    #129003

    Purpose

    pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-myc-Rem2 RNAiR2 short

    Plasmid

    #51596

    Purpose

    expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    silent mutations at shRNA 1 and 2 recognition sit…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-myc-Rem2 R236A/L263A

    Plasmid

    #51589

    Purpose

    expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R236A and L263A; silent mutations at shRNA 1 and …

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-myc-Rem2 S69A/S334A

    Plasmid

    #51588

    Purpose

    expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S69A and S334A; silent mutations at shRNA 1 and 2…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 4xHRE_RE:RedF

    Plasmid

    #178314

    Purpose

    HRE RedFirefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    HRE RedFirefly reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti CMV V5-LUC Blast (w567-1)

    Plasmid

    #21474

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    V5-LUC
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DCIP

    Plasmid

    #193860

    Purpose

    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    NLS-mCherry-GFP1-10
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti PGK V5-LUC Neo (w623-2)

    Plasmid

    #21471

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    V5-LUC
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pax5

    Plasmid

    #35003

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Paired box protein Pax-5 (Pax5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH6HTC_IRP1-Halo

    Plasmid

    #175305

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IRP1 (ACO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA)

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NbV5-HALOTAG

    Plasmid

    #201482

    Purpose

    Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-HALOTAG
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    3xP3

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScalps Luciferase Zsgreen

    Plasmid

    #184784

    Purpose

    Lentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoter

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Insert

    Firefly Luciferase and Zsgreen
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mNG-sfTq2ox

    Plasmid

    #124219

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGreen_dualluc_3'UTR_sensor

    Plasmid

    #55206

    Purpose

    Dual-luciferase reporter for microRNA targeting in plants

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Luciferase; Plant transient expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZmUBQ:LUC

    Plasmid

    #131860

    Purpose

    Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMOD_C'5802

    Plasmid

    #176879

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10:Luc+:RbcsE9
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-MAT2A

    Plasmid

    #164820

    Purpose

    Expresses MAT2A in E. coli BL21(DE3)

    Depositor

    Vern Schramm

    Article

    Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

    Insert

    Methionine Adenosyltransferase 2A (MAT2A Human)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

    Plasmid

    #168192

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-LaG6-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ORF57 Pr pGL4.16

    Plasmid

    #120378

    Purpose

    Firefly luciferase reporter with KSHV ORF57 early gene promoter

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF57 Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    ORF57 Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET12a-AL-09 FL

    Plasmid

    #47081

    Depositor

    Marina Ramirez-Alvarado

    Article

    McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

    Insert

    AL-09 (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX330-sgRNA_Dgcr8_1

    Plasmid

    #73525

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

39. mNG-sfTq2

    Plasmid

    #124218

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pColE1_sgRNA_M4NS_AmpR

    Plasmid

    #119156

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRL_yEGFP_hc

    Plasmid

    #98746

    Purpose

    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Insert

    Yeast-enhanced green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    synthetic

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV.pCry1(408).DIO.Luciferase

    Plasmid

    #192623

    Purpose

    Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

    Depositor

    Michael Hastings

    Article

    Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

    Insert

    luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mNG-mTq2

    Plasmid

    #124217

    Purpose

    Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-mTq2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. p663-UBC-miniTurbo-V5-CDK16_IDG-K

    Plasmid

    #158173

    Purpose

    Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK16 (CDK16 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

    Plasmid

    #168189

    Purpose

    HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pENTR-R4R3-3xmEGFP-C

    Plasmid

    #113751

    Purpose

    Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmEGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. PB-HD118

    Plasmid

    #172655

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD118A/pegRNA-HD118B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge