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pMET7-EDHFR-FKBP12

Plasmid

#194454

Purpose

Mammalian expression vector that expresses the EDHFR protein with FKBP12 fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

eDHFR

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-human FKBP12 (HK174)

Plasmid

#206459

Purpose

FKBP12 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔJ (Δ14-82)

Plasmid

#205719

Purpose

Mammalian expression of DNAJC5-ΔJ (Δ14-82)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

Δ14-82

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L115R

Plasmid

#205708

Purpose

Mammalian expression of DNAJC5 L115R

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L116Δ

Plasmid

#205709

Purpose

Mammalian expression of DNAJC5 L116Δ

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

L116Δ

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaRII-Cterm-myc

Plasmid

#185505

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

RII domain and C-term deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pWP001

Plasmid

#178596

Purpose

pAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiT

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

CBD-SmBiT/LgBiT

Expression

Bacterial

Promoter

Pxyl

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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CMV BARK R106A p2A mCherry

Plasmid

#117688

Purpose

CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs R106A peptide

Tags

HA / FLAG

Expression

Mammalian

Mutation

R106A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN

Plasmid

#135517

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld-VN

Plasmid

#135520

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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hsa-cdk2 3'UTR sicheck2

Plasmid

#121125

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR (CDK2 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-Ib

Plasmid

#121896

Purpose

Plasmid for making zebrafish apoA-Ib in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA1b (apoa1b Zebrafish)

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoBb.2

Plasmid

#121899

Purpose

Plasmid for making zebrafish apoBb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBb.2 (apobb.2 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.3

Plasmid

#121905

Purpose

Plasmid for making zebrafish apoA-IVb.3 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoa4b.3 (apoa4b.3 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVa

Plasmid

#121902

Purpose

Plasmid for making zebrafish apoA-IVa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA4a (apoa4a Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-Ia

Plasmid

#121895

Purpose

Plasmid for making zebrafish apoA-Ia in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoA-Ia (apoa1a Zebrafish)

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-2/3

Plasmid

#91551

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-2/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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