We narrowed to 2,218 results for: dcas9

1. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEC819

   Plasmid

   #190815

   Purpose

   CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

   Depositor

   James Chappell

   Article

   Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   dCas9-XTEN-rpoA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   XTEN (linker), followed by the N-terminal domain …

   Expression

   Mutation

   Promoter

   SP30

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC005

   Plasmid

   #171140

   Purpose

   For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   XylS-Pm
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. dSp-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99673

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-dSa-VPR mini.-2X snRP1

   Plasmid

   #99688

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. dSp-VP64-p65(150-261)-RTA(125-190)

    Plasmid

    #99674

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-P65(151-261)-RTA(125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK660

    Plasmid

    #242914

    Purpose

    Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    PspF-λN22 (pspF Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23107

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK071

    Plasmid

    #242907

    Purpose

    Constitutive expression of dCas9 and PspF-λN22

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    PspF-λN22 (pspF Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23107

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK320

    Plasmid

    #242906

    Purpose

    aTc-inducible expression of dCas9-AsiA

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    AsiA (asiA Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    TetR-Ptet

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK321

    Plasmid

    #242912

    Purpose

    aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    MCP-SoxS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23107

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. dSp-VP64-p65(100-261)-RTA(75-190)

    Plasmid

    #99672

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-p65(101-261)-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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