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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)

Plasmid Kit

#1000000175

Purpose

Plasmids and Gateway entry vectors for fluorescent sensors of diverse physiological parameters in cells.

Depositor

Adam Cohen

Application

Gene Expression and Labeling

Vector Type

Lentiviral, Mammalian Expression

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
EB3-mNeonGreen

Plasmid

#98881

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-ABCG2-IRES-Neo

Plasmid

#25983

Depositor

Ren-he Xu

Article

Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

Insert

ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)

Use

Lentiviral

Tags

EF1-apha promoter and IRES-Neo

Expression

Mammalian

Mutation

None

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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DT0

Plasmid

#80418

Purpose

Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMC037-HisMS2_PLP_pac

Plasmid

#128233

Purpose

Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Flag-YAP-S94A

Plasmid

#33102

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S94A

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
PGKp-GFP-TAZ

Plasmid

#174176

Purpose

Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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