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Showing: 4701 - 4750 of 6656 results

  2. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

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  3. pVC-FUS-R521C

    Plasmid
    #175067
    Purpose
    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    changed Arginin 521 to Cystein; mVenus: C-Termina…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175067-plasmid-map-sequence-id-362347

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  4. Desmoplakin II tension sensor (F40-based)

    Plasmid
    #118715
    Purpose
    The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118715-plasmid-map-sequence-id-230444

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  5. pCC336

    Plasmid
    #110167
    Purpose
    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43E, T55E, T62E.…
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110167-plasmid-map-sequence-id-238139

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  6. pCC335

    Plasmid
    #110166
    Purpose
    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43A, T55A, T62A.…
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110166-plasmid-map-sequence-id-235342

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  7. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  8. Desmoplakin II tension sensor (FL-based)

    Plasmid
    #118721
    Purpose
    The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118721-plasmid-map-sequence-id-230467

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  9. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #118725
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118725-plasmid-map-sequence-id-230441

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  10. Desmoplakin II no force control (F40-based)

    Plasmid
    #118716
    Purpose
    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118716-plasmid-map-sequence-id-230464

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  11. LADL Bridge + Promoter Only Target

    Plasmid
    #127669
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    127669-plasmid-map-sequence-id-249167

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  12. Desmoplakin II no force control (FL-based)

    Plasmid
    #118724
    Purpose
    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118724-plasmid-map-sequence-id-230471

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  15. pT22Ubm4Eng2bKRPC(#493)

    Plasmid
    #184091
    Purpose
    cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:
    Insert
    4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    4xMyc
    Expression
    Mutation
    ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184091-plasmid-map-sequence-id-363977

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  17. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118726-plasmid-map-sequence-id-230443

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  24. Reb_206 aka PAS629

    Plasmid
    #71234
    Purpose
    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry
    Depositor
    Pamela Silver
    Article
    Polka et al ACS Synth Biol. 2016 Feb 3.
    Insert
    Reb locus
    Use
    Tags
    mNeonGreen to second copy RebB, soluble mCherry a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    71234-plasmid-map-sequence-id-244339

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  25. pJA291

    Plasmid
    #74487
    Purpose
    eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP
    Depositor
    Andrew Fire
    Article
    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do
    Insert
    puroR-mCherry-T2A-eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74487-plasmid-map-sequence-id-140489

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  37. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  40. pDM1424

    Plasmid
    #207424
    Purpose
    Extrachromosomal expression HSPC300-GFP and PkgE-PH-mCherry
    Depositor
    Rob Kay
    Article
    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755
    Inserts
    HSPC300
    PkgE
    Use
    Unspecified
    Tags
    GFP and mCherry
    Expression
    Mutation
    aa 1–100 (PH domain) only
    Promoter
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    207424-plasmid-map-sequence-id-414332

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Showing: 4701 - 4750 of 6656 results