Addgene

We narrowed to 2,124 results for: AP1

Showing: 1881 - 1900 of 2124 results

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35R H43R SUMO1

Plasmid

#221063

Purpose

Expression of H35R H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R H43R double mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H35R, H43R

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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small ubiquitin-like modifier 1 (SUMO1)

Plasmid

#221052

Purpose

Expression of small ubiquitin-like modifier 1 (SUMO1) protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ HA BEX3

Plasmid

#231004

Purpose

Transient overexpression of human BEX3 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX3 (BEX3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-acatenin.22-906

Plasmid

#186460

Purpose

Expresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 CI

Plasmid

#227191

Purpose

Inducible lentiviral expression of CI (Catalytically Inactive) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

H309N

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0700

Plasmid

#143725

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PAXBP1 (PAXBP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-γ1

Plasmid

#197078

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-1 γ1 (Ap1g1 Mouse)

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-mDia1-GBD (1-305 aa) in pEGFPN1

Plasmid

#187280

Purpose

Express EGFP-mDia1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

mDia1 (Diaph1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (1-305aa)

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PX458_ARID4B_1

Plasmid

#101078

Purpose

Encodes gRNA for 3' target of human ARID4B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID4B (ARID4B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-H122Y

Plasmid

#33085

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H122Y

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Use

Tags

FLAG peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-DAXX

Plasmid

#169726

Purpose

Mammalian expression construct of GFP tagged full length DAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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