We narrowed to 3,948 results for: SPL

1. 2Flag-pDEST-N

   Plasmid

   #118371

   Purpose

   Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC_HA_2xPP7-VP64

   Plasmid

   #68418

   Purpose

   Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   PP7(deltaFG)
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM378

   Plasmid

   #173174

   Purpose

   Expresses C9-1 gRNA-12xMBS from hU6 promoter

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   C9-1 targeting gRNA-12xMBS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL_U2AF1_WT_mCherry

   Plasmid

   #84017

   Purpose

   expression of U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP_sfCherry2(11)_HP1

   Plasmid

   #82607

   Purpose

   Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_HP1b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pRRL_U2AF1_S34F_mCherry

   Plasmid

   #84019

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34F

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-GFP1-10-pDEST-N

   Plasmid

   #118368

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaVα2δ1

   Plasmid

   #26575

   Depositor

   Diane Lipscombe

   Article

   Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

   Insert

   Cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. NIR-AG

   Plasmid

   #210728

   Purpose

   NIR light-activated GSDMD

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   NIR-AG (GSDMD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2Flag-pDEST-C

    Plasmid

    #118372

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACUH-GFP11x7-mCherry-α-tubulin

    Plasmid

    #70218

    Purpose

    Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11x7-mCherry-α-tubulin
    

    Use

    Tags

    GFP11x7 and mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-GFP1-10-pDEST-C

    Plasmid

    #118369

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140220

    Purpose

    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1
    

    Use

    CRISPR

    Tags

    miniVPR-mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG SMG1 WT

    Plasmid

    #199573

    Purpose

    Expresses FLAG-tagged WT SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Use

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-pEF1a-tTAc

    Plasmid

    #172123

    Purpose

    Constitutive expression of InteinC-tTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2HA-pDEST-C

    Plasmid

    #118374

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SFG.CNb30_opt.IRES.eGFP

    Plasmid

    #22493

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    L124T; K125-LA-Ins

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

    Plasmid

    #162037

    Purpose

    Background-free mRNA labeling tripartite superfolder GFP system

    Depositor

    Hye Yoon Park

    Article

    Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

    Insert

    GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge