We narrowed to 10,627 results for: otos

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pTomo-Ras

Plasmid

#26292

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

HRAS-V12 (HRAS Human)

Use

Lentiviral

Tags

Flag

Mutation

G12V

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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PM-ABKAR

Plasmid

#61506

Purpose

Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Lyn kinase sequence (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pBa.Kif5c 1-559.GFP

Plasmid

#45059

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif5c (Kif5c Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

Chicken Beta Actin

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII(K42M)

Plasmid

#165433

Purpose

Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(K42M)

Tags

tdTomato

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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3349 pSFFV-neo Bcl-2

Plasmid

#8769

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCALNL-NaChBac

Plasmid

#125580

Purpose

Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

NaChBac

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_WT

Plasmid

#190743

Purpose

For expression of wild-type rat SERT in mammalian cells

Depositor

Gary Rudnick

Article

Singh et al bioRxiv 2022.06.13.495991

Insert

SLC6A4 Serotonin Transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G261A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
CMV-tdTomato-P2A-paCaMKII(T286A)

Plasmid

#165432

Purpose

Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(T286A)

Tags

tdTomato

Expression

Mammalian

Mutation

T286A: Autophosphorylation deficient mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPAiRFP2-N1

Plasmid

#44273

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Expression

Mammalian

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:AVRa1-V1

Plasmid

#132357

Purpose

Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

AVRa1-V1 ∆SP with stop codon

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT003

Plasmid

#182713

Purpose

aTc inducible dCasRx-IF3

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

3x(GGGS)-Escherichia Coli Initiation Factor 3

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT005

Plasmid

#182715

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Ensconsin-18-mEos2

Plasmid

#99230

Purpose

Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

mEos2

Tags

Ensconsin (MAP7)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PDK1-FLAG-LOV*

Plasmid

#202205

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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