Addgene

We narrowed to 31,164 results for: uros

Showing: 1881 - 1900 of 31164 results

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4

Plasmid

#112844

Purpose

transient/stable overexpression of the N-terminal and central domains of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N + C1 domains of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMY-mTurquoise2-P2A-PuroR-HA

Plasmid

#163344

Purpose

Retroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection marker

Depositor

Teunis Geijtenbeek, Louis Ates

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mTurquoise2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

Plasmid

#159287

Purpose

Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT7 (ID. 5) (KAT7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-G368A_VA

Plasmid

#141330

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G368A in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G368A (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

G368A

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) cRel shRNA2

Plasmid

#22510

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA2 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-Puro-FH-AGO2-S831E

Plasmid

#91997

Purpose

Expresses FLAG-HA-AGO2 (S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF9

Plasmid

#117159

Purpose

Target site AATTATCACAAAGAGGACAGG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Serine 102 to Alanine

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pQC V5 HuR.wt IRES Puro

Plasmid

#110386

Purpose

γ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-G287A_VA

Plasmid

#141328

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G287A in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

G287A

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cc-TARDBP-W385G_VA

Plasmid

#141331

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-W385G in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-W385G (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

W385G

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pAG181 NeuroD 3'UTR mut

Plasmid

#12039

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N14 3'UTR mut (NEUROD1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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