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pReg-THP4

Plasmid

#190671

Purpose

Expresses an evolved tetrahydropapaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

THP4

Use

Synthetic Biology

Promoter

J23103

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-sGFP-Vrg4

Plasmid

#160904

Purpose

Expresses GFP-Vrg4 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

VRG4

Tags

GFP

Expression

Yeast

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSL5200 (pDonor_ISGst3)

Plasmid

#210875

Purpose

Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

ISGst3

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MDA5A

Plasmid

#42394

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MDA5A (IFIH1 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
PE-GsI-IIC-RT

Plasmid

#181808

Purpose

PE-GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-GsI-IIC-RT

Use

CRISPR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Xba1

Plasmid

#203300

Purpose

For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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scFv-M-MLV-RT

Plasmid

#181809

Purpose

scFv-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV_RT

Use

Lentiviral

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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36 p flk1

Plasmid

#13061

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

flk1 (kdrl Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
petRNA-FANCF-v3'

Plasmid

#181803

Purpose

petRNA FANCF v3'

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

ribozyme-petRNA

Use

CRISPR

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-pegRNA

Plasmid

#181811

Purpose

ms2 incorporated pegRNA-1.1

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MS2-pegRNA

Use

CRISPR

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBS mScx (CT#404)

Plasmid

#13958

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (Scx Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-mMaroon

Plasmid

#217008

Purpose

lentiviral plasmid for gRNA cloning with selectable mMaroon expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSL4672 (pEffector_TnpB3,ωRNA)

Plasmid

#210872

Purpose

Expresses GstTnpB3 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB3

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRN104

Plasmid

#127231

Purpose

35S::AFR5::35S terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Mpdelta

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac FPSC

Plasmid

#1926

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

posterior sex combs (Psc Fly)

Tags

flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBS cScx 3'UTR (CT#485)

Plasmid

#13957

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (SCX Chicken)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pReg-ROTU4

Plasmid

#190669

Purpose

Expresses an evolved rotundine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

ROTU4

Use

Synthetic Biology

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGfOMT1-GEN4

Plasmid

#190612

Purpose

Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GfOMT-GEN4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-GLAU4

Plasmid

#190667

Purpose

Expresses an evolved glaucine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GLAU4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-PAP4

Plasmid

#190668

Purpose

Expresses an evolved papaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

PAP4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-NOS4

Plasmid

#190670

Purpose

Expresses an evolved noscapine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

NOS4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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HA GST F5A deltaCT S6K1 pRK5

Plasmid

#8467

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta CT p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pNM261

Plasmid

#128411

Purpose

Vanillic acid inducible production of lysostaphin. LsyT with no signaling peptide for secretion.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

Lysostaphin (epr Staphylococcus simulans)

Expression

Bacterial

Promoter

Pspank(V)

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_HuB

Plasmid

#65764

Purpose

expresses RFP-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
HA GST deltaCT S6K1 pRK5

Plasmid

#8468

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta CT p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCTK131

Plasmid

#248494

Purpose

Encodes PBAD-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

PBAD-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCTK130

Plasmid

#248493

Purpose

Encodes J23101-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

J23101-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCTK132

Plasmid

#248495

Purpose

Encodes PTet-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

PTet-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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