We narrowed to 23,428 results for: CRISPR

1. pZNF257.1.0-gDNA

   Plasmid

   #112390

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF257

   Depositor

   Kevin White

   Insert

   ZNF257 (ZNF257 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A-1x2

   Plasmid

   #58766

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCB320

   Plasmid

   #89359

   Purpose

   3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   puromycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pxpr_502-wt-ltr

   Plasmid

   #182647

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

   Depositor

   Jakob Reiser

   Article

   Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI152-pLenti-SiT-Cas12a

   Plasmid

   #128405

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JME4580

   Plasmid

   #129659

   Purpose

   HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiSAMPHv2

    Plasmid

    #167934

    Purpose

    All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid

    #117134

    Purpose

    All-in-one expression vector for Cas9 and gRNA against CD44

    Depositor

    Kosuke Yusa

    Insert

    gCD44 v2 (Cd44 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EF1alpha-dCas9-p300_Blast

    Plasmid

    #192653

    Purpose

    3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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