We narrowed to 12,012 results for: pcdna

1. pcDNA3-DN-hCUL2-FLAG

   Plasmid

   #15819

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin2 (CUL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N427

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Gβγ-BERKY3

   Plasmid

   #158219

   Purpose

   BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. LRP4-Flag/pCDNA3.1

   Plasmid

   #197291

   Purpose

   Plasmid expressing an antigen targeting LRP4 (extracellular)

   Depositor

   Steve Burden

   Insert

   LRP4 (Lrp4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4-AMBRA1-3xFLAG

   Plasmid

   #174157

   Purpose

   Vector for expression of 3xFLAG-tagged wild-type AMBRA1

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 HA-SUMO2-Q90P

   Plasmid

   #36448

   Purpose

   Expresses HA-Sumo2Q90P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutated Q90P

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

   Plasmid

   #169332

   Purpose

   Nuclear expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag9 (H2BC21 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyn-dInPAkt

   Plasmid

   #181834

   Purpose

   Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dInPAkt
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-Flag-BRD4-7A

   Plasmid

   #90006

   Purpose

   expresses 7A mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 484S to 484A, 488S to 488A, 492S to 492A,…

   Promoter

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA HA HP1 alpha

    Plasmid

    #24078

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1.Gαi1-LgB91

    Plasmid

    #134342

    Purpose

    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi1-LgB91 (GNAI1 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Rho*-BERKY3

    Plasmid

    #158220

    Purpose

    BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 19, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA3-FLAG-Lin28B

    Plasmid

    #51373

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyn-dPlcR

    Plasmid

    #181832

    Purpose

    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/ZMYM2-FGFR1

    Plasmid

    #167486

    Purpose

    Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

    Insert

    ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Gαq*-BERKY3

    Plasmid

    #158217

    Purpose

    BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA-HaloGFP-Na2.4

    Plasmid

    #228471

    Purpose

    Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

    Depositor

    Robert Campbell

    Article

    Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

    Insert

    HaloGFP-Na2.4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 (-) hYAP 1-2alpha

    Plasmid

    #124143

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-SOX4

    Plasmid

    #110360

    Purpose

    Mammalian expression of FLAG-tagged SOX4

    Depositor

    Carlos Moreno

    Article

    Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacking 5' UTR

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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