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2.2

Plasmid

#34885

Depositor

Irvin Chen

Article

Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

Insert

Sindbis virus envelope protein

Use

Lentiviral

Expression

Mammalian

Mutation

SGN UTR in E1 domain

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRAM28

Plasmid

#234314

Purpose

pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 2

Use

Synthetic Biology

Promoter

P. CymRC

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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BiP-mCherry

Plasmid

#62233

Purpose

Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mCherry-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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sgTrack-mCherry

Plasmid

#114013

Purpose

Empty sgRNA expression vector with co-expression of mCherry reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-TET3G-T2A-Puro

Plasmid

#158067

Purpose

Encodes the Tet3G transactivator and puromycin resistance.

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

pCAG-TET3G-T2A-Puro

Use

AAV

Tags

puromycin reistance gene

Expression

Mammalian

Promoter

pCAG

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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sgTrack-GFP

Plasmid

#114012

Purpose

Empty sgRNA expression vector with co-expression of TurboGFP reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry

Plasmid

#214143

Purpose

Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCherry

Expression

Mammalian

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM4-C-19

Plasmid

#54167

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM4

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pdCas9-NED

Plasmid

#109358

Purpose

Mammalian vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR

Tags

dCas9, SV40 NLS, 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA LIP

Plasmid

#15739

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM6-C-19

Plasmid

#54171

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits