We narrowed to 6,255 results for: tTA

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pCAS_gpyrG2

Plasmid

#90277

Purpose

(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg2)

Use

A. niger

Expression

Bacterial

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90285

Purpose

BB2 with split pyrG marker for homologous integration with linker AB (negative control)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD434

Plasmid

#63757

Purpose

EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.

Depositor

James Kaper

Article

Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

Insert

eae probe

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pSS63

Plasmid

#47951

Purpose

rtTA positive feedback loop with single tet operator in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

T3

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Tcrb-V13 in situ probe

Plasmid

#45622

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Tcrb-V13 in situ probe (Tcrb Mouse)

Use

In situ

Mutation

fragment contains bp# 533-1058 of X67128

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
Sgf73(1-106)/PET32a

Plasmid

#26962

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sgf73(1-106) (SGF73 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab8b_1

Plasmid

#26708

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast

Plasmid

#26655

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HRV-3C Protease-mCherry

Plasmid

#243792

Purpose

Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HRV-3C protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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HIR WT

Plasmid

#24049

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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PKD1-F (pCI-PKD1-Flag)

Plasmid

#21369

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Tags

2xFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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