We narrowed to 2,475 results for: T2A

1. pMCB619

   Plasmid

   #171011

   Purpose

   CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   U6:sgRNA_Puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PXL

   Plasmid

   #75349

   Purpose

   PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-PM

   Plasmid

   #197386

   Purpose

   To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-syn-GCaMP6m-XC

   Plasmid

   #118975

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP6m

   Depositor

   Xiaodong Liu

   Article

   Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

   Insert

   GCaMP6m-XC
   

   Use

   AAV

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT-GEM(0)

   Plasmid

   #62891

   Purpose

   Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pCaMKII-tTAc

   Plasmid

   #172125

   Purpose

   Expression of InteinC-tTAC in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, tTAc
   

   Use

   AAV

   Promoter

   CaMKII

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH4542

   Plasmid

   #200779

   Purpose

   Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GCaMP6
   

   Tags

   mNeptune

   Expression

   Worm

   Promoter

   Ptwk-40

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLV-rtTA-zeo

   Plasmid

   #183233

   Purpose

   Lentiviral vector expressing the rtTa gene

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   rtTA-T2A-Zeo
   

   Use

   Lentiviral

   Promoter

   UBC

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA1_NANOG

   Plasmid

   #64153

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-pEF1a-rtTAc

    Plasmid

    #172121

    Purpose

    Constitutive expression of InteinC-rtTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-rtTAc
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK3_027

    Plasmid

    #123742

    Purpose

    Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VP16::PYLcs-T2A-GAL4DBD::ABIcs
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPN460

    Plasmid

    #137878

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ1371-sgYAP-2

    Plasmid

    #121423

    Purpose

    sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgYAP-2
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA4_NANOG

    Plasmid

    #64156

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA3_NANOG

    Plasmid

    #64155

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA2_NANOG

    Plasmid

    #64154

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Split PS Intein gal4-vp16

    Plasmid

    #242030

    Purpose

    Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein gal4-vp16
    

    Expression

    Mammalian

    Mutation

    pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIRC5-Split PS Intein-N tTA

    Plasmid

    #242033

    Purpose

    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-N tTA
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    The CMV promoter was replaced with the BIRC5 prom…

    Promoter

    hTERT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. phTERT-Split PS Intein-N tTA

    Plasmid

    #242034

    Purpose

    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-N tTA
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    The CMV promoter was replaced with the hTERT prom…

    Promoter

    hTERT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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