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GPR149-Tango
Plasmid#66324PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR149 (GPR149 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-wt
Plasmid#15263DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-wt
Plasmid#15290DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
NC15 pGLUE CUL3
Plasmid#36970DepositorInsertCUL3 (CUL3 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR149
Plasmid#106049PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)DepositorInsertOpto-GPR149 (GPR149 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
iYFP-Parkin Plasmid
Plasmid#197107PurposeExpresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.DepositorInsertparkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
UseRetroviralTagsYFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2267
Plasmid#142189PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARNT2 (ARNT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-mut (super repressor)
Plasmid#15264DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationSerine 32 to Alanine, Serine 36 to AlaninePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Anti-WAVE1/SCAR [K91/36R]
Plasmid#128629PurposeMammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.DepositorInsertanti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROG2loop
Plasmid#215033PurposeMammalian expression of human TWIST1 (with NEUROG2 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P4-P1R:PER36modp
Plasmid#128431PurposeGateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).DepositorInsertPEROXISADE36 (AT3G50990 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPremature stop codons introduced into the Xero1 (…PromoterPEROXISADE36Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_NFKBIA_WT
Plasmid#81833PurposeGateway Donor vector containing NFKBIA ,part of the Target Accelerator Plasmid Collection.DepositorInsertNFKBIA (NFKBIA Human)
UseGateway entry vectorTagsExpressionMutationS76PPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NFKBIA_p.P137S
Plasmid#81367PurposeGateway Donor vector containing NFKBIA , part of the Target Accelerator Plasmid Collection.DepositorInsertNFKBIA (NFKBIA Human)
UseGateway entry vectorTagsExpressionMutationS76P; P137SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NFKBIA_WT
Plasmid#81256PurposeGateway Donor vector containing NFKBIA , part of the Target Accelerator Plasmid Collection.DepositorInsertNFKBIA (NFKBIA Human)
UseGateway entry vectorTagsExpressionMutationS76PPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…ExpressionMutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCVneo-HA-ER-Hoxb8
Plasmid#222291PurposeThe most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.DepositorUseRetroviralTagsHAExpressionMammalianMutationThe estrogen receptor hormone binding domain cont…PromoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
