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We narrowed to 32,933 results for: tes

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Showing: 1921 - 1940 of 32933 results

  7. pJK048

    Plasmid
    #75230
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    75230-plasmid-map-sequence-id-142240

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  8. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  9. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  10. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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  11. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  12. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

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  13. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-GFAP104-mCherry (AAV5)

    Viral Prep
    #58909-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP104-mCherry (#58909). In addition to the viral particles, you will also receive purified pAAV-GFAP104-mCherry plasmid DNA. GFAP104-driven mCherry expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc
    Promoter
    GFAP
    Tags
    mCherry
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)

    Viral Prep
    #100896-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Philip Haydon
    Article
    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi
    Promoter
    GFA104 (GFAP promoter)
    Tags
    EGFP
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-EF1a-4mtGCaMP6f-DIO (AAV1)

    Viral Prep
    #179529-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-4mtGCaMP6f-DIO (#179529). In addition to the viral particles, you will also receive purified pAAV-EF1a-4mtGCaMP6f-DIO plasmid DNA. EF1a-driven, Cre-dependent expression of mitochondria-targeted GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Maarten Kole
    Article
    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:
    Promoter
    EF1a
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-EF1a-mitoGFP-DIO (AAV2)

    Viral Prep
    #174112-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-EF1a-mitoGFP-DIO (#174112). In addition to the viral particles, you will also receive purified pAAV-EF1a-mitoGFP-DIO plasmid DNA. EF1a-driven, Cre-dependent expression of mitochondria-targeted GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Maarten Kole
    Article
    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:
    Promoter
    EF1a
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-GFAP104-ChR2-mCherry (AAV5)

    Viral Prep
    #58892-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP104-ChR2-mCherry (#58892). In addition to the viral particles, you will also receive purified pAAV-GFAP104-ChR2-mCherry plasmid DNA. GFAP -driven, expression of humanized ChR2 H134R mutant fused to mCherry, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc
    Promoter
    GFAP104
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV.hSyn1.Twitch2B.WPRE.SV40 (AAV1)

    Viral Prep
    #100040-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn1.Twitch2B.WPRE.SV40 (#100040). In addition to the viral particles, you will also receive purified pAAV.hSyn1.Twitch2B.WPRE.SV40 plasmid DNA. Synapsin-driven expression of (FRET)-based calcium biosensor Twitch2B. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Oliver Griesbeck
    Article
    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm
    Promoter
    Syn
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1921 - 1940 of 32933 results